Immunoblot

The Immunoblot is highly specific in detecting whether an antibody response exists against a particular pathogen.

  • Evidence of lgM antibodies usually provides an indication of a recently acquired infection. However, lgM antibodies can also persist in the event of chronic sicknesses over long periods of time.
  • In the earliest cases, lgG antibodies occur months after an infection and represent a previous antigen antibody contact. In the context of clinical symptoms, IgG antibodies may provide information on the late stage of borreliosis.

The Immunoblot is composed of blot strips upon which separate single antigens of the pathogens are affixed. These are capable of binding antibodies (lgG and lgM respectively) and are dyed via chemical reaction for easy visualisation. The stripe normally also contains VisE (a surface marker), which has a high level of sensitivity to the organism.

Material: 1 x Serum tubes (centrifuge after blood drawing)

Turnaround time: Two days (Note: Taking into account the required pre- and post-analytical work, the report is created in about one week)

Further reading and studies can be found here.

 

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