This laboratory method will be available from 01.02.2016!
The PCR (Polymerase Chain Reaction) is a molecular biological method for the direct detection of a pathogen’s nucleic acids (DNA/RNA) in a sample. The detectability of pathogen-DNA/RNA in blood or tissue indicates the (temporary) presence of the pathogen. A positive PCR result therefore provides direct proof of the presence of an infection with the detected pathogen.
However, it should be noted that the presence of a pathogen in a sample and thereby the detectability of pathogen-specific nucleic acids depends on the pathomechanism and also varies with the stage of disease.
Prior to the actual PCR-Test the entire DNA/RNA is isolated from a patient’s blood or tissue sample. These nucleic acids are then used as starting material for the requested pathogen-specific PCR-Test.
During the PCR reaction specific oligonucleotides (primer) are used for each pathogen which only bind to a sequences of a gene of the pathogen enabling amplification of this gene and subsequent detection of the pathogen.
Thus a very high specificity is achieved. Advanced technique, i.e. the nested PCR, real-time PCR or qPCR, achieve the highest sensitivity for the detection of pathogens.
For the detection of Lyme disease pathogens we developed our own in-house PCR testing system, which is based on multiple consecutive PCR reactions and which detects different pathogen genes with high sensitivity and specificity. The BCA-lab is therefore one of the few laboratories that is able to detect Borrelia miyamotoi. The spectrum of molecular biological pathogen detection is completed by a PCR-based Bartonella-Assay.
Material for blood analysis: 4 x EDTA-tubes, 1 x serum tube and 1 x CPDA-tube
Material for tissue/biopsy analysis: 1-2 pieces of tissue (> 1mm³) in sterile tubes filled with 0,9% sodium chloride solution at 4°C; for a longer transport (> 24 hr) immediately freeze tissue samples in sterile tubes without liquid at -20°C or -80°C
Turnaround time: 3 days (Note: Taking into account the required pre- and post-analytical work, the report is created in about 2 weeks)
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